› 3kl3

X-ray diffraction
2.33Å resolution

Crystal structure of Ligand bound XynC

Released:
DOI: 10.2210/pdb3kl3/pdb
PDB entry 3kl3 coloured by chain, front view.
PDB entry 3kl3 coloured by chain, side view.
PDB entry 3kl3 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Ligand bound structures of a glycosyl hydrolase family 30 glucuronoxylan xylanohydrolase.
St John FJ, Hurlbert JC, Rice JD, Preston JF, Pozharski E
J Mol Biol 407 92-109 (2011)
PMID: 21256135

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosyl links in some glucuronoarabinoxylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucuronoxylanase XynC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 401 amino acids
Theoretical weight: 45.43 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45070 (Residues: 33-422; Coverage: 100%)
Gene names: BSU18150, xynC, ynfF
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GCU 2 x GCU
Ligand DHI 2 x DHI
Ligand BDP 1 x BDP
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21212
Unit cell:
a: 137.869Å b: 192.733Å c: 65.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.248
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties.
Nordberg Karlsson et al. (2018)
4 more

2 mentions without citation

A plasmid borne, functionally novel glycoside hydrolase family 30 subfamily 8 endoxylanase from solventogenic Clostridium.
St John et al. (2018)
1 more