› 3kkq

X-ray diffraction
1.2Å resolution

Crystal structure of M-Ras P40D in complex with GDP

Released:
DOI: 10.2210/pdb3kkq/pdb
PDB entry 3kkq coloured by chain, front view.
PDB entry 3kkq coloured by chain, side view.
PDB entry 3kkq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for conformational dynamics of GTP-bound Ras protein.
Shima F, Ijiri Y, Muraoka S, Liao J, Ye M, Araki M, Matsumoto K, Yamamoto N, Sugimoto T, Yoshikawa Y, Kumasaka T, Yamamoto M, Tamura A, Kataoka T
J Biol Chem 285 22696-705 (2010)
PMID: 20479006

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein M-Ras Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08989 (Residues: 1-178; Coverage: 86%)
Gene names: Mras, Xras
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 64.947Å b: 70.754Å c: 43.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.192
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Direct small-molecule inhibitors of KRAS: from structural insights to mechanism-based design.
Ostrem et al. (2016)
18 more

2 mentions without citation

Structural basis for conformational dynamics of GTP-bound Ras protein.
Shima et al. (2010)
1 more