› 3kki

X-ray diffraction
1.8Å resolution

PLP-Dependent Acyl-CoA transferase CqsA

Released:
DOI: 10.2210/pdb3kki/pdb
PDB entry 3kki coloured by chain, front view.
PDB entry 3kki coloured by chain, side view.
PDB entry 3kki coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA.
Kelly RC, Bolitho ME, Higgins DA, Lu W, Ng WL, Jeffrey PD, Rabinowitz JD, Semmelhack MF, Hughson FM, Bassler BL
Nat Chem Biol 5 891-5 (2009)
PMID: 19838203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CAI-1 autoinducer synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 45.81 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KM65 (Residues: 1-389; Coverage: 100%)
Gene names: VC_A0523, cqsA
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PLP 2 x PLP
Ligand MG 2 x MG
Ligand SO4 2 x SO4
Ligand DTT 2 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 70.621Å b: 177.443Å c: 70.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.234
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Quorum sensing signal-response systems in Gram-negative bacteria.
Papenfort et al. (2016)
21 more

6 mentions without citation

Anatomy of enzyme channels.
Pravda et al. (2014)
5 more