› 3ken

X-ray diffraction
2.5Å resolution

Human Eg5 in complex with S-trityl-L-cysteine

Released:
DOI: 10.2210/pdb3ken/pdb
PDB entry 3ken coloured by chain, front view.
PDB entry 3ken coloured by chain, side view.
PDB entry 3ken coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric drug discrimination is coupled to mechanochemical changes in the kinesin-5 motor core.
Kim ED, Buckley R, Learman S, Richard J, Parke C, Worthylake DK, Wojcik EJ, Walker RA, Kim S
J Biol Chem 285 18650-61 (2010)
PMID: 20299460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52732 (Residues: 1-369; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand ZZD 1 x ZZD
Ligand KEN 1 x KEN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: I213
Unit cell:
a: 157.93Å b: 157.93Å c: 157.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Kinesin-5: cross-bridging mechanism to targeted clinical therapy.
Wojcik et al. (2013)
2 more

16 mentions without citation

Recent findings and future directions for interpolar mitotic kinesin inhibitors in cancer therapy.
Myers et al. (2016)
15 more