› 3k36

X-ray diffraction
2.2Å resolution

Crystal Structure of B/Perth Neuraminidase

Released:
DOI: 10.2210/pdb3k36/pdb
PDB entry 3k36 coloured by chain, front view.
PDB entry 3k36 coloured by chain, side view.
PDB entry 3k36 coloured by chain, top view.
Source organism: Influenza B virus (B/Perth/211/2001)
Primary publication:
Structural and functional basis of resistance to neuraminidase inhibitors of influenza B viruses.
Oakley AJ, Barrett S, Peat TS, Newman J, Streltsov VA, Waddington L, Saito T, Tashiro M, McKimm-Breschkin JL
J Med Chem 53 6421-31 (2010)
PMID: 20695427

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 43.82 KDa
Source organism: Influenza B virus (B/Perth/211/2001)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3S340 (Residues: 70-466; Coverage: 85%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 2 x EDO
Ligand GOL 4 x GOL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I4
Unit cell:
a: 87.64Å b: 87.64Å c: 197.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Influenza neuraminidase inhibitors: antiviral action and mechanisms of resistance.
McKimm-Breschkin JL. (2013)
9 more

11 mentions without citation

SETting the Stage for Cancer Development: SETD2 and the Consequences of Lost Methylation.
Fahey et al. (2017)
10 more