› 3k2u

X-ray diffraction
2.35Å resolution

Crystal structure of HGFA in complex with the allosteric inhibitory antibody Fab40

Released:
DOI: 10.2210/pdb3k2u/pdb
PDB entry 3k2u coloured by chain, front view.
PDB entry 3k2u coloured by chain, side view.
PDB entry 3k2u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unraveling the allosteric mechanism of serine protease inhibition by an antibody.
Ganesan R, Eigenbrot C, Wu Y, Liang WC, Shia S, Lipari MT, Kirchhofer D
Structure 17 1614-1624 (2009)
PMID: 20004165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211911 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hepatocyte growth factor activator long chain Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 27.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 408-655; Coverage: 40%)
Gene name: HGFAC
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor activator short chain Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q04756 (Residues: 373-407; Coverage: 6%)
Gene name: HGFAC
Antibody, Fab fragment, Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody, Fab fragment, Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 38.944Å b: 48.933Å c: 96.034Å
α: 98.1° β: 95.01° γ: 103.89°
R-values:
R R work R free
0.239 0.239 0.29
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

8 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
7 more

7 mentions without citation

Allostery and population shift in drug discovery.
Kar et al. (2010)
6 more