› 3k08

X-ray diffraction
1.62Å resolution

Crystal Structure of CNG mimicking NaK mutant, NaK-NTPP, Na+ complex

Released:
DOI: 10.2210/pdb3k08/pdb
PDB entry 3k08 coloured by chain, front view.
PDB entry 3k08 coloured by chain, side view.
PDB entry 3k08 coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Structural studies of ion permeation and Ca2+ blockage of a bacterial channel mimicking the cyclic nucleotide-gated channel pore.
Derebe MG, Zeng W, Li Y, Alam A, Jiang Y
Proc Natl Acad Sci U S A 108 592-7 (2011)
PMID: 21187429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.61 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 20-66, 68-110; Coverage: 78%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand MPD 6 x MPD
Ligand NA 10 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4
Unit cell:
a: 67.78Å b: 67.78Å c: 89.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.227
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The physiology of mechanoelectrical transduction channels in hearing.
Fettiplace et al. (2014)
5 more

1 mention without citation

Structural studies of ion permeation and Ca2+ blockage of a bacterial channel mimicking the cyclic nucleotide-gated channel pore.
Derebe et al. (2011)