› 3jxu

X-ray diffraction
2.14Å resolution

Crystal structure of the human 70kDa heat shock protein 1A (Hsp70-1) ATPase domain in complex with ADP and inorganic phosphate

Released:
DOI: 10.2210/pdb3jxu/pdb
PDB entry 3jxu coloured by chain, front view.
PDB entry 3jxu coloured by chain, side view.
PDB entry 3jxu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.
Wisniewska M, Karlberg T, Lehtiö L, Johansson I, Kotenyova T, Moche M, Schüler H
PLoS One 5 e8625 (2010)
PMID: 20072699

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock 70 kDa protein 1A Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 45.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV8 (Residues: 1-387; Coverage: 60%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 46.013Å b: 63.298Å c: 144.197Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.262
Expression system: Escherichia coli

Quick links

Citations

11 review citations

HSPA8/HSC70 chaperone protein: structure, function, and chemical targeting.
Stricher et al. (2013)
10 more

9 mentions without citation

Targeting Allosteric Control Mechanisms in Heat Shock Protein 70 (Hsp70).
Li et al. (2016)
8 more