› 3jvv

X-ray diffraction
2.6Å resolution

Crystal Structure of P. aeruginosa PilT with bound AMP-PCP

Released:
DOI: 10.2210/pdb3jvv/pdb
PDB entry 3jvv coloured by chain, front view.
PDB entry 3jvv coloured by chain, side view.
PDB entry 3jvv coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
P. aeruginosa PilT structures with and without nucleotide reveal a dynamic type IV pilus retraction motor.
Misic AM, Satyshur KA, Forest KT
J Mol Biol 400 1011-21 (2010)
PMID: 20595000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV pilus retractation ATPase PilT Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 356 amino acids
Theoretical weight: 39.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P24559 (Residues: 1-344; Coverage: 100%)
Gene names: PA0395, pilT
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACP 3 x ACP
Ligand MG 3 x MG
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 108.483Å b: 119.552Å c: 185.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.291
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
10 more

14 mentions without citation

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
13 more