› 3jul

X-ray diffraction
2.4Å resolution

Crystal structure of Listeria innocua D-Tagatose-6-Phosphate Kinase bound with substrate

Released:
DOI: 10.2210/pdb3jul/pdb
PDB entry 3jul coloured by chain, front view.
PDB entry 3jul coloured by chain, side view.
PDB entry 3jul coloured by chain, top view.
Source organism: Listeria innocua
Entry authors: Satyanarayana L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tagatose-6-phosphate kinase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.34 KDa
Source organism: Listeria innocua
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q929S5 (Residues: 2-310; Coverage: 100%)
Gene name: lin2199
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand TA6 1 x TA6
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 84.307Å b: 44.24Å c: 79.116Å
α: 90° β: 104.65° γ: 90°
R-values:
R R work R free
0.226 0.226 0.267
Expression system: Escherichia coli BL21(DE3)

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