› 3j8a

Electron Microscopy
3.7Å resolution

Structure of the F-actin-tropomyosin complex

Released:
DOI: 10.2210/pdb3j8a/pdb
PDB entry 3j8a coloured by chain, front view.
PDB entry 3j8a coloured by chain, side view.
PDB entry 3j8a coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the F-actin-tropomyosin complex.
von der Ecken J, Müller M, Lehman W, Manstein DJ, Penczek PA, Raunser S
Nature 519 114-7 (2015)
PMID: 25470062
Related structures: EMD-6124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
tropomyosin alpha-1 Chains: F, G
Molecule details ›
Chains: F, G
Length: 135 amino acids
Theoretical weight: 11.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand ADP 5 x ADP
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-6124
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Actin and Actin-Binding Proteins.
Pollard TD. (2016)
43 more

30 mentions without citation

Regulated methionine oxidation by monooxygenases.
Manta et al. (2017)
29 more