› 3iav

X-ray diffraction
1.75Å resolution

Propionyl-CoA Carboxylase Beta Subunit, D422V

Released:
DOI: 10.2210/pdb3iav/pdb
PDB entry 3iav coloured by chain, front view.
PDB entry 3iav coloured by chain, side view.
PDB entry 3iav coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Crystal structures and mutational analyses of acyl-CoA carboxylase beta subunit of Streptomyces coelicolor.
Arabolaza A, Shillito ME, Lin TW, Diacovich L, Melgar M, Pham H, Amick D, Gramajo H, Tsai SC
Biochemistry 49 7367-76 (2010)
PMID: 20690600

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Propionyl-CoA carboxylase complex B subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 530 amino acids
Theoretical weight: 57.21 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X4K7 (Residues: 1-530; Coverage: 100%)
Gene name: pccB
Sequence domains: Carboxyl transferase domain
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P63
Unit cell:
a: 171.531Å b: 171.531Å c: 75.068Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.233
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
5 more

1 mention without citation

Crystal structures and mutational analyses of acyl-CoA carboxylase beta subunit of Streptomyces coelicolor.
Arabolaza et al. (2010)