› 3i5o

X-ray diffraction
1.5Å resolution

The X-ray crystal structure of a thermophilic cellobiose binding protein bound with cellopentaose

Released:
DOI: 10.2210/pdb3i5o/pdb
PDB entry 3i5o coloured by chain, front view.
PDB entry 3i5o coloured by chain, side view.
PDB entry 3i5o coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold.
Cuneo MJ, Beese LS, Hellinga HW
J Biol Chem 284 33217-23 (2009)
PMID: 19801540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Solute-binding protein family 5 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 592 amino acids
Theoretical weight: 68.73 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WXN8 (Residues: 22-606; Coverage: 100%)
Gene name: TM_0031
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 62.11Å b: 101.46Å c: 108.29Å
α: 90° β: 94.07° γ: 90°
R-values:
R R work R free
0.205 0.204 0.225
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
Eitinger et al. (2011)
1 more

2 mentions without citation

Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold.
Cuneo et al. (2009)
1 more