› 3i32

X-ray diffraction
2.8Å resolution

Dimeric structure of a Hera helicase fragment including the C-terminal RecA domain, the dimerization domain, and the RNA binding domain

Released:
DOI: 10.2210/pdb3i32/pdb
PDB entry 3i32 coloured by chain, front view.
PDB entry 3i32 coloured by chain, side view.
PDB entry 3i32 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
The Thermus thermophilus DEAD box helicase Hera contains a modified RNA recognition motif domain loosely connected to the helicase core.
Rudolph MG, Klostermeier D
RNA 15 1993-2001 (2009)
PMID: 19710183

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.42 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 218-517; Coverage: 58%)
Gene name: TT_C1895
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand BR 2 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 64.96Å b: 64.96Å c: 153.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.253 0.292
Expression system: Escherichia coli

Quick links

Citations

4 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
3 more

5 mentions without citation

A homology model of the pore domain of a voltage-gated calcium channel is consistent with available SCAM data.
Bruhova et al. (2010)
4 more