› 3hx6

X-ray diffraction
2.1Å resolution

Crystal structure of Pseudomonas aeruginosa PilY1 C-terminal domain

Released:
DOI: 10.2210/pdb3hx6/pdb
PDB entry 3hx6 coloured by chain, front view.
PDB entry 3hx6 coloured by chain, side view.
PDB entry 3hx6 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure analysis reveals Pseudomonas PilY1 as an essential calcium-dependent regulator of bacterial surface motility.
Orans J, Johnson MD, Coggan KA, Sperlazza JR, Heiniger RW, Wolfgang MC, Redinbo MR
Proc Natl Acad Sci U S A 107 1065-70 (2010)
PMID: 20080557

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV pilus biogenesis factor PilY1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 570 amino acids
Theoretical weight: 61.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVM8 (Residues: 613-1161; Coverage: 49%)
Gene names: PA4554, pilY1
Sequence domains: Neisseria PilC beta-propeller domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 64Å b: 108Å c: 159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.196 0.235
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
11 more

9 mentions without citation

Crystal structure analysis reveals Pseudomonas PilY1 as an essential calcium-dependent regulator of bacterial surface motility.
Orans et al. (2010)
8 more