› 3hnj

X-ray diffraction
2Å resolution

Crystal structure of the Zn-induced tetramer of the engineered cyt cb562 variant RIDC-2

Released:
DOI: 10.2210/pdb3hnj/pdb
PDB entry 3hnj coloured by chain, front view.
PDB entry 3hnj coloured by chain, side view.
PDB entry 3hnj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal templated design of protein interfaces.
Salgado EN, Ambroggio XI, Brodin JD, Lewis RA, Kuhlman B, Tezcan FA
Proc Natl Acad Sci U S A 107 1827-32 (2010)
PMID: 20080561

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 48.448Å b: 65.316Å c: 70.773Å
α: 90° β: 104.7° γ: 90°
R-values:
R R work R free
0.228 0.224 0.272
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
24 more

2 mentions without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)
1 more