› 3hka

X-ray diffraction
1.9Å resolution

Crystal structure of uronate isomerase from Bacillus halodurans complexed with zinc and D-Fructuronate

Released:
DOI: 10.2210/pdb3hka/pdb
PDB entry 3hka coloured by chain, front view.
PDB entry 3hka coloured by chain, side view.
PDB entry 3hka coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily.
Nguyen TT, Fedorov AA, Williams L, Fedorov EV, Li Y, Xu C, Almo SC, Raushel FM
Biochemistry 48 8879-90 (2009)
PMID: 19678710

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BH0493 protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 427 amino acids
Theoretical weight: 50.01 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KFI6 (Residues: 1-427; Coverage: 100%)
Gene name: BH0493
Structure domains: uronate isomerase, domain 2, chain A

Ligands and Environments

4 bound ligands:
Ligand FIX 3 x FIX
Ligand CO3 3 x CO3
Ligand ZN 4 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P4122
Unit cell:
a: 132.596Å b: 132.596Å c: 195.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals.
Mehtiö et al. (2016)
1 more

2 mentions without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)
1 more