› 3hde

X-ray diffraction
1.95Å resolution

Crystal structure of full-length endolysin R21 from phage 21

Released:
DOI: 10.2210/pdb3hde/pdb
PDB entry 3hde coloured by chain, front view.
PDB entry 3hde coloured by chain, side view.
PDB entry 3hde coloured by chain, top view.
Source organism: Enterobacteria phage P21
Primary publication:
Regulation of a muralytic enzyme by dynamic membrane topology.
Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC
Nat Struct Mol Biol 16 1192-4 (2009)
PMID: 19881499

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAR-endolysin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 165 amino acids
Theoretical weight: 18.04 KDa
Source organism: Enterobacteria phage P21
Expression system: Escherichia coli
UniProt:
  • Canonical: P27359 (Residues: 1-165; Coverage: 100%)
Gene name: R
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 78.249Å b: 94.814Å c: 97.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Phage lysis: three steps, three choices, one outcome.
Young R. (2014)
6 more

5 mentions without citation

Regulation of a muralytic enzyme by dynamic membrane topology.
Sun et al. (2009)
4 more