› 3hcg

X-ray diffraction
1.82Å resolution

Structure of the C-terminal domain (MsrB) of Neisseria meningitidis PilB (reduced form)

Released:
DOI: 10.2210/pdb3hcg/pdb
PDB entry 3hcg coloured by chain, front view.
PDB entry 3hcg coloured by chain, side view.
PDB entry 3hcg coloured by chain, top view.
Source organism: Neisseria meningitidis serogroup A
Primary publication:
Methionine sulfoxide reductase B displays a high level of flexibility.
Ranaivoson FM, Neiers F, Kauffmann B, Boschi-Muller S, Branlant G, Favier F
J Mol Biol 394 83-93 (2009)
PMID: 19733575

Function and Biology Details

Reactions catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide methionine sulfoxide reductase MsrA/MsrB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16.49 KDa
Source organism: Neisseria meningitidis serogroup A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JWM8 (Residues: 377-522; Coverage: 28%)
Gene names: NMA0290, msrAB, pilB
Sequence domains: SelR domain
Structure domains: Peptide methionine sulfoxide reductase.

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 43.663Å b: 117.232Å c: 137.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.185 0.235
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Reversible and irreversible protein glutathionylation: biological and clinical aspects.
Cooper et al. (2011)
6 more

2 mentions without citation

Molecular Mechanisms of the Methionine Sulfoxide Reductase System from Neisseria meningitidis.
Boschi-Muller S. (2018)
1 more