› 3hbj

X-ray diffraction
2.1Å resolution

Structure of UGT78G1 complexed with UDP

Released:
DOI: 10.2210/pdb3hbj/pdb
PDB entry 3hbj coloured by chain, front view.
PDB entry 3hbj coloured by chain, side view.
PDB entry 3hbj coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Crystal structures of glycosyltransferase UGT78G1 reveal the molecular basis for glycosylation and deglycosylation of (iso)flavonoids.
Modolo LV, Li L, Pan H, Blount JW, Dixon RA, Wang X
J Mol Biol 392 1292-302 (2009)
PMID: 19683002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavonoid 3-O-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 50.22 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli
UniProt:
  • Canonical: A6XNC6 (Residues: 1-454; Coverage: 100%)
Gene names: GT83F, UGT78G1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 85.12Å b: 77.77Å c: 70.025Å
α: 90° β: 93.36° γ: 90°
R-values:
R R work R free
0.209 0.209 0.281
Expression system: Escherichia coli

Quick links

Citations

12 review citations

First-pass metabolism via UDP-glucuronosyltransferase: a barrier to oral bioavailability of phenolics.
Wu et al. (2011)
11 more

4 mentions without citation

Understanding substrate selectivity of human UDP-glucuronosyltransferases through QSAR modeling and analysis of homologous enzymes.
Dong et al. (2012)
3 more