› 3h3d

X-ray diffraction
2.3Å resolution

Drosophila Pumilio RNA binding domain (Puf domain)

Released:
DOI: 10.2210/pdb3h3d/pdb
PDB entry 3h3d coloured by chain, front view.
PDB entry 3h3d coloured by chain, side view.
PDB entry 3h3d coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of Pumilio reveals similarity between RNA and peptide binding motifs.
Edwards TA, Pyle SE, Wharton RP, Aggarwal AK
Cell 105 281-9 (2001)
PMID: 11336677

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maternal protein pumilio Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 323 amino acids
Theoretical weight: 37.01 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P25822 (Residues: 1093-1411; Coverage: 21%)
Gene names: CG9755, pum
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P63
Unit cell:
a: 94.58Å b: 94.58Å c: 229.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.298
Expression system: Escherichia coli

Quick links

Citations

36 review citations

RNA-binding proteins and post-transcriptional gene regulation.
Glisovic et al. (2008)
35 more

3 mentions without citation

The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation.
Loedige et al. (2014)
2 more