› 3gw8

X-ray diffraction
1.93Å resolution

Crystal structure of phosphoglyceromutase from Burkholderia pseudomallei with vanadate and glycerol

Released:
DOI: 10.2210/pdb3gw8/pdb
PDB entry 3gw8 coloured by chain, front view.
PDB entry 3gw8 coloured by chain, side view.
PDB entry 3gw8 coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Primary publication:
An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Davies DR, Staker BL, Abendroth JA, Edwards TE, Hartley R, Leonard J, Kim H, Rychel AL, Hewitt SN, Myler PJ, Stewart LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1044-50 (2011)
PMID: 21904048

Function and Biology Details

Reaction catalysed: 
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.96 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JWH7 (Residues: 1-249; Coverage: 100%)
Gene names: BURPS1710b_0662, gpmA
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

3 bound ligands:
Ligand VO4 4 x VO4
Ligand GOL 2 x GOL
Ligand PG4 4 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 45.175Å b: 49.112Å c: 62.59Å
α: 106.42° β: 91.18° γ: 107.61°
R-values:
R R work R free
0.189 0.187 0.227
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Oxidative stress activates SIRT2 to deacetylate and stimulate phosphoglycerate mutase.
Xu et al. (2014)
8 more

1 mention without citation

An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Davies et al. (2011)