› 3gpq

X-ray diffraction
2Å resolution

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
NTP + H(2)O = NDP + phosphate
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-185033 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Non-structural protein 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 168 amino acids
Theoretical weight: 18.64 KDa
Source organism: Chikungunya virus strain S27-African prototype
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JUX6 (Residues: 1334-1493; Coverage: 7%)
Sequence domains: Macro domain
Structure domains: Leucine Aminopeptidase, subunit E, domain 1
RNA (5'-R(*AP*AP*A)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 3 nucleotides
Theoretical weight: 943 Da
Source organism: Chikungunya virus strain S27-African prototype
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P31
Unit cell:
a: 86.824Å b: 86.824Å c: 81.316Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

44 review citations

PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes.
Gupte et al. (2017)
43 more

4 mentions without citation

AlphaFold2 protein structure prediction: Implications for drug discovery.
Borkakoti et al. (2023)
3 more