› 3gnp

X-ray diffraction
1.8Å resolution

Crystal Structure of a Rice Os3BGlu6 Beta-Glucosidase with Octyl-Beta-D-Thio-Glucoside

Released:
DOI: 10.2210/pdb3gnp/pdb
PDB entry 3gnp coloured by chain, front view.
PDB entry 3gnp coloured by chain, side view.
PDB entry 3gnp coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides.
Seshadri S, Akiyama T, Opassiri R, Kuaprasert B, Cairns JK
Plant Physiol 151 47-58 (2009)
PMID: 19587102

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase 6 Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 55.32 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L7J2 (Residues: 38-521; Coverage: 100%)
Gene names: BGLU6, LOC_Os03g11420, Os03g0212800
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand SOG 1 x SOG
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 57.185Å b: 91.093Å c: 111.359Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.207
Expression system: Escherichia coli

Quick links

Citations

3 review citations

β-Glucosidases.
Ketudat Cairns et al. (2010)
2 more

2 mentions without citation

Effects of Filament Extrusion, 3D Printing and Hot-Pressing on Electrical and Tensile Properties of Poly(Lactic) Acid Composites Filled with Carbon Nanotubes and Graphene.
Spinelli et al. (2019)
1 more