3gib

X-ray diffraction
2.4Å resolution

Crystal Structure of the Complex of the E. coli Hfq with Poly(A)

Released:
Source organism: Escherichia coli K-12
Primary publication:
Structure of Escherichia coli Hfq bound to polyriboadenylate RNA.
Proc Natl Acad Sci U S A 106 19292-7 (2009)
PMID: 19889981

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-141441 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Hfq Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 68 amino acids
Theoretical weight: 7.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6X3 (Residues: 2-69; Coverage: 67%)
Gene names: JW4130, b4172, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.
5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3' Chain: H
Molecule details ›
Chain: H
Length: 9 nucleotides
Theoretical weight: 2.92 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 66.534Å b: 88.96Å c: 39.635Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.259
Expression systems:
  • Escherichia coli
  • Not provided