› 3gh9

X-ray diffraction
1.69Å resolution

Crystal structure of EDTA-treated BdbD (Oxidised)

Released:
DOI: 10.2210/pdb3gh9/pdb
PDB entry 3gh9 coloured by chain, front view.
PDB entry 3gh9 coloured by chain, side view.
PDB entry 3gh9 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure and biophysical properties of Bacillus subtilis BdbD. An oxidizing thiol:disulfide oxidoreductase containing a novel metal site.
Crow A, Lewin A, Hecht O, Carlsson Möller M, Moore GR, Hederstedt L, Le Brun NE
J Biol Chem 284 23719-33 (2009)
PMID: 19535335

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disulfide bond formation protein D Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 23.04 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32218 (Residues: 30-222; Coverage: 100%)
Gene names: BSU33480, bdbD, yvgV
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand UNX 1 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 38.55Å b: 43.57Å c: 54.56Å
α: 90° β: 107.38° γ: 90°
R-values:
R R work R free
0.198 0.195 0.25
Expression system: Escherichia coli

Quick links

Citations

9 review citations

How proteins form disulfide bonds.
Depuydt et al. (2011)
8 more

1 mention without citation

Crystal structure and biophysical properties of Bacillus subtilis BdbD. An oxidizing thiol:disulfide oxidoreductase containing a novel metal site.
Crow et al. (2009)