› 3gee

X-ray diffraction
2.95Å resolution

Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID

Released:
DOI: 10.2210/pdb3gee/pdb
PDB entry 3gee coloured by chain, front view.
PDB entry 3gee coloured by chain, side view.
PDB entry 3gee coloured by chain, top view.
Source organism: Chlorobaculum tepidum
Primary publication:
Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy.
Meyer S, Böhme S, Krüger A, Steinhoff HJ, Klare JP, Wittinghofer A
PLoS Biol 7 e1000212 (2009)
PMID: 19806182

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA modification GTPase MnmE Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 52.17 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KAS1 (Residues: 1-473; Coverage: 100%)
Gene names: CT2084, mnmE, thdF, trmE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand FON 1 x FON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4122
Unit cell:
a: 130.804Å b: 130.804Å c: 200.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Biosynthesis and function of posttranscriptional modifications of transfer RNAs.
El Yacoubi et al. (2012)
7 more

5 mentions without citation

Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy.
Meyer et al. (2009)
4 more