› 3ge9

X-ray diffraction
2.61Å resolution

A Structurally Atypical ThyX from Corynebacterium glutamicum NCHU 87078 Is Not Required for Thymidylate Biosynthesis

Released:
DOI: 10.2210/pdb3ge9/pdb
PDB entry 3ge9 coloured by chain, front view.
PDB entry 3ge9 coloured by chain, side view.
PDB entry 3ge9 coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Entry authors: Wang W-C, Liu J-S, Chang C-M

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-154028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent thymidylate synthase Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.79 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P40111 (Residues: 1-250; Coverage: 100%)
Gene names: Cgl1972, cg2162, thyX
Sequence domains: Thymidylate synthase complementing protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4222
Unit cell:
a: 96.322Å b: 96.322Å c: 64.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.284
Expression system: Escherichia coli

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