› 3fyt

X-ray diffraction
2.58Å resolution

Crystal structure of Bacillus pumilus acetyl xylan esterase S181A mutant in complex with beta-D-xylopyranose

Released:
DOI: 10.2210/pdb3fyt/pdb
PDB entry 3fyt coloured by chain, front view.
PDB entry 3fyt coloured by chain, side view.
PDB entry 3fyt coloured by chain, top view.
Source organism: Bacillus pumilus
Entry authors: Krastanova I, Cassetta A, Lamba D

Function and Biology Details

Reaction catalysed: 
An acetic ester + H(2)O = an alcohol + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl xylan esterase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, L, M, N
Length: 320 amino acids
Theoretical weight: 36.1 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K5F2 (Residues: 1-320; Coverage: 100%)
Gene name: axe
Sequence domains: Acetyl xylan esterase (AXE1)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 34 x CL
Ligand XYP 6 x XYP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 87.186Å b: 167.986Å c: 144.471Å
α: 90° β: 91.17° γ: 90°
R-values:
R R work R free
0.188 0.188 0.241
Expression system: Escherichia coli

Quick links