› 3fyr

X-ray diffraction
1.97Å resolution

Crystal structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis

Released:
DOI: 10.2210/pdb3fyr/pdb
PDB entry 3fyr coloured by chain, front view.
PDB entry 3fyr coloured by chain, side view.
PDB entry 3fyr coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state.
Jacques DA, Streamer M, Rowland SL, King GF, Guss JM, Trewhella J, Langley DB
Acta Crystallogr D Biol Crystallogr 65 574-81 (2009)
PMID: 19465772

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-182152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation inhibitor sda Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 48 amino acids
Theoretical weight: 5.69 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WY62 (Residues: 7-52; Coverage: 89%)
Gene names: BSU25690, sda
Sequence domains: Sporulation inhibitor A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 36.972Å b: 36.972Å c: 167.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.304
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfalls.
Jacques et al. (2010)
1 more

1 mention without citation

Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state.
Jacques et al. (2009)