› 3ft3

X-ray diffraction
1.95Å resolution

Crystal Structure of the minor histocompatibility peptide HA-1His in complex with HLA-A2

Released:
DOI: 10.2210/pdb3ft3/pdb
PDB entry 3ft3 coloured by chain, front view.
PDB entry 3ft3 coloured by chain, side view.
PDB entry 3ft3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Steric hindrance and fast dissociation explain the lack of immunogenicity of the minor histocompatibility HA-1Arg Null allele.
Spierings E, Gras S, Reiser JB, Mommaas B, Almekinders M, Kester MG, Chouquet A, Le Gorrec M, Drijfhout JW, Ossendorp F, Housset D, Goulmy E
J Immunol 182 4809-16 (2009)
PMID: 19342659

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137823 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Minor histocompatibility antigen HA-1 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92619 (Residues: 137-145; Coverage: 1%)
Gene names: ARHGAP45, HMHA1, KIAA0223

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 51.47Å b: 79.75Å c: 54.21Å
α: 90° β: 111.02° γ: 90°
R-values:
R R work R free
0.184 0.182 0.231
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Exploiting T cells specific for human minor histocompatibility antigens for therapy of leukemia.
Bleakley et al. (2011)
9 more

1 mention without citation

Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope.
Song et al. (2017)