› 3fsv

X-ray diffraction
2.3Å resolution

Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAHAAAM)

Released:
DOI: 10.2210/pdb3fsv/pdb
PDB entry 3fsv coloured by chain, front view.
PDB entry 3fsv coloured by chain, side view.
PDB entry 3fsv coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Metal-binding loop length and not sequence dictates structure.
Sato K, Li C, Salard I, Thompson AJ, Banfield MJ, Dennison C
Proc Natl Acad Sci U S A 106 5616-21 (2009)
PMID: 19299503

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.71 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 99%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I222
Unit cell:
a: 53.836Å b: 55.819Å c: 74.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.257 0.308
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Enhancement of catalysis and functional expression of a bacterial laccase by single amino acid replacement.
Nasoohi et al. (2013)
12 more

2 mentions without citation

Linking in domain-swapped protein dimers.
Baiesi et al. (2016)
1 more