› 3fpd

X-ray diffraction
2.4Å resolution

G9a-like protein lysine methyltransferase inhibition by BIX-01294

Released:
DOI: 10.2210/pdb3fpd/pdb
PDB entry 3fpd coloured by chain, front view.
PDB entry 3fpd coloured by chain, side view.
PDB entry 3fpd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for G9a-like protein lysine methyltransferase inhibition by BIX-01294.
Chang Y, Zhang X, Horton JR, Upadhyay AK, Spannhoff A, Liu J, Snyder JP, Bedford MT, Cheng X
Nat Struct Mol Biol 16 312-7 (2009)
PMID: 19219047

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(9) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(9)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase EHMT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 30.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9B1 (Residues: 1006-1266; Coverage: 20%)
Gene names: EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Sequence domains:
Structure domains: SET domain

Ligands and Environments

3 bound ligands:
Ligand SAH 2 x SAH
Ligand Q4A 2 x Q4A
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 75.3Å b: 95.2Å c: 101.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.262
Expression system: Escherichia coli

Quick links

Citations

65 review citations

Epigenetic protein families: a new frontier for drug discovery.
Arrowsmith et al. (2012)
64 more

22 mentions without citation

EZH2: biology, disease, and structure-based drug discovery.
Tan et al. (2014)
21 more