› 3flb

X-ray diffraction
1.8Å resolution

RifR - Type II thioesterase from Rifamycin NRPS/PKS biosynthetic pathway - Form 2

Released:
DOI: 10.2210/pdb3flb/pdb
PDB entry 3flb coloured by chain, front view.
PDB entry 3flb coloured by chain, side view.
PDB entry 3flb coloured by chain, top view.
Source organism: Amycolatopsis mediterranei
Primary publication:
Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway.
Claxton HB, Akey DL, Silver MK, Admiraal SJ, Smith JL
J Biol Chem 284 5021-9 (2009)
PMID: 19103602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase thioesterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 29.26 KDa
Source organism: Amycolatopsis mediterranei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BUF9 (Residues: 1-259; Coverage: 100%)
Gene name: rifR
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 38.938Å b: 62.497Å c: 82.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.239
Expression system: Escherichia coli

Quick links

Citations

5 review citations

PKS and NRPS release mechanisms.
Du et al. (2010)
4 more

9 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
8 more