› 3fev

X-ray diffraction
1.3Å resolution

Crystal structure of the chimeric muscarinic toxin MT7 with loop 1 from MT1.

Released:
DOI: 10.2210/pdb3fev/pdb
PDB entry 3fev coloured by chain, front view.
PDB entry 3fev coloured by chain, side view.
PDB entry 3fev coloured by chain, top view.
Source organism: Dendroaspis angusticeps
Primary publication:
Engineering of three-finger fold toxins creates ligands with original pharmacological profiles for muscarinic and adrenergic receptors.
Fruchart-Gaillard C, Mourier G, Blanchet G, Vera L, Gilles N, Ménez R, Marcon E, Stura EA, Servent D
PLoS One 7 e39166 (2012)
PMID: 22720062

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Muscarinic toxin 1; Muscarinic toxin 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 65 amino acids
Theoretical weight: 7.38 KDa
Source organism: Dendroaspis angusticeps
Expression system: Not provided
UniProt:
  • Canonical: Q8QGR0 (Residues: 22-25, 48-86; Coverage: 66%)
  • Canonical: P81030 (Residues: 5-26; Coverage: 33%)
Sequence domains: Snake toxin and toxin-like protein
Structure domains: CD59

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 27.14Å b: 71.975Å c: 89.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.233
Expression system: Not provided

Quick links

Citations

3 review citations

The three-finger toxin fold: a multifunctional structural scaffold able to modulate cholinergic functions.
Kessler et al. (2017)
2 more

3 mentions without citation

Transplanting supersites of HIV-1 vulnerability.
Zhou et al. (2014)
2 more