› 3fb7

X-ray diffraction
3.3Å resolution

Open KcsA potassium channel in the presence of Rb+ ion

Released:
DOI: 10.2210/pdb3fb7/pdb
PDB entry 3fb7 coloured by chain, front view.
PDB entry 3fb7 coloured by chain, side view.
PDB entry 3fb7 coloured by chain, top view.
Source organisms:
Entry authors: Cuello LG, Jogini V, Cortes DM, Pan AC, Gagnon DH, Cordero-Morales JF, Chakrapani S, Roux B, Perozo E

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-209445 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody fab fragment heavy chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
antibody fab fragment light chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 104 amino acids
Theoretical weight: 10.93 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 21-124; Coverage: 65%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand RB 4 x RB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4
Unit cell:
a: 157.799Å b: 157.799Å c: 73.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.255
Expression system: Escherichia coli

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