› 3f4i

X-ray diffraction
1.95Å resolution

Crystal Structure of LeuT bound to L-selenomethionine and sodium

Released:
DOI: 10.2210/pdb3f4i/pdb
PDB entry 3f4i coloured by chain, front view.
PDB entry 3f4i coloured by chain, side view.
PDB entry 3f4i coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
A competitive inhibitor traps LeuT in an open-to-out conformation.
Singh SK, Piscitelli CL, Yamashita A, Gouaux E
Science 322 1655-61 (2008)
PMID: 19074341

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.08 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand MSE 1 x MSE
Ligand BOG 5 x BOG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 89.777Å b: 86.703Å c: 81.692Å
α: 90° β: 95.85° γ: 90°
R-values:
R R work R free
0.194 0.192 0.218
Expression system: Escherichia coli

Quick links

Citations

55 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
54 more

3 mentions without citation

Structure, Function, and Modulation of γ-Aminobutyric Acid Transporter 1 (GAT1) in Neurological Disorders: A Pharmacoinformatic Prospective.
Zafar et al. (2018)
2 more