› 3f1b

X-ray diffraction
2.4Å resolution

The crystal structure of a TetR-like transcriptional regulator from Rhodococcus sp. RHA1.

Released:
DOI: 10.2210/pdb3f1b/pdb
PDB entry 3f1b coloured by chain, front view.
PDB entry 3f1b coloured by chain, side view.
PDB entry 3f1b coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Tan K, Evdokimova E, Kudritska M, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.3 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0S658 (Residues: 1-199; Coverage: 100%)
Gene name: RHA1_ro05197
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 53.991Å b: 53.991Å c: 129.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.278
Expression system: Escherichia coli BL21

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