› 3evx

X-ray diffraction
2.54Å resolution

Crystal structure of the human E2-like ubiquitin-fold modifier conjugating enzyme 1 (Ufc1). Northeast Structural Genomics Consortium target HR41

Released:
DOI: 10.2210/pdb3evx/pdb
PDB entry 3evx coloured by chain, front view.
PDB entry 3evx coloured by chain, side view.
PDB entry 3evx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NMR and X-RAY structures of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1) reveal structural and functional conservation in the metazoan UFM1-UBA5-UFC1 ubiquination pathway.
Liu G, Forouhar F, Eletsky A, Atreya HS, Aramini JM, Xiao R, Huang YJ, Abashidze M, Seetharaman J, Liu J, Rost B, Acton T, Montelione GT, Hunt JF, Szyperski T
J Struct Funct Genomics 10 127-36 (2009)
PMID: 19101823

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-fold modifier-conjugating enzyme 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 175 amino acids
Theoretical weight: 20.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3C8 (Residues: 1-167; Coverage: 100%)
Gene names: CGI-126, HSPC155, UFC1
Sequence domains: Ubiquitin-fold modifier-conjugating enzyme 1
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand SCN 4 x SCN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 41.368Å b: 64.537Å c: 66.422Å
α: 90.06° β: 89.95° γ: 105.18°
R-values:
R R work R free
0.234 0.233 0.279
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The UFMylation System in Proteostasis and Beyond.
Gerakis et al. (2019)
7 more

5 mentions without citation

Ubiquitin-like Protein Conjugation: Structures, Chemistry, and Mechanism.
Cappadocia et al. (2018)
4 more