› 3es9

X-ray diffraction
3.4Å resolution

NADPH-Cytochrome P450 Reductase in an Open Conformation

Released:
DOI: 10.2210/pdb3es9/pdb
PDB entry 3es9 coloured by chain, front view.
PDB entry 3es9 coloured by chain, side view.
PDB entry 3es9 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure and function of an NADPH-cytochrome P450 oxidoreductase in an open conformation capable of reducing cytochrome P450.
Hamdane D, Xia C, Im SC, Zhang H, Kim JJ, Waskell L
J Biol Chem 284 11374-84 (2009)
PMID: 19171935

Function and Biology Details

Reaction catalysed: 
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH--cytochrome P450 reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 618 amino acids
Theoretical weight: 70.26 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00388 (Residues: 57-678; Coverage: 91%)
Gene name: Por
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 3 x FAD

Cofactor: Ligand NAP 2 x NAP

Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 109.1Å b: 93Å c: 125.7Å
α: 90° β: 100° γ: 90°
R-values:
R R work R free
0.219 0.219 0.279
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
15 more

25 mentions without citation

The interaction of microsomal cytochrome P450 2B4 with its redox partners, cytochrome P450 reductase and cytochrome b(5).
Im et al. (2011)
24 more