› 3es3

X-ray diffraction
2.8Å resolution

Directing Noble Metal Ion Chemistry within a Designed Ferritin Protein. The Complex with Gold ions. Ferritin H8-H9x Mutant

Released:
DOI: 10.2210/pdb3es3/pdb
PDB entry 3es3 coloured by chain, front view.
PDB entry 3es3 coloured by chain, side view.
PDB entry 3es3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Directing noble metal ion chemistry within a designed ferritin protein.
Butts CA, Swift J, Kang SG, Di Costanzo L, Christianson DW, Saven JG, Dmochowski IJ
Biochemistry 47 12729-39 (2008)
PMID: 18991401

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 21.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 1-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand AU 4 x AU
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: F432
Unit cell:
a: 181.564Å b: 181.564Å c: 181.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.275
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
7 more

2 mentions without citation

Ferritin: A Promising Nanoreactor and Nanocarrier for Bionanotechnology.
Mohanty et al. (2022)
1 more