› 3eip

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF COLICIN E3 IMMUNITY PROTEIN: AN INHIBITOR TO A RIBOSOME-INACTIVATING RNASE

Released:
DOI: 10.2210/pdb3eip/pdb
PDB entry 3eip coloured by chain, front view.
PDB entry 3eip coloured by chain, side view.
PDB entry 3eip coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Crystal structure of colicin E3 immunity protein: an inhibitor of a ribosome-inactivating RNase.
Li C, Zhao D, Djebli A, Shoham M
Structure 7 1365-72 (1999)
PMID: 10574790

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin E3 immunity protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 9.78 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P02984 (Residues: 2-85; Coverage: 99%)
Gene names: ceiC, imm, immB
Sequence domains: Cloacin immunity protein
Structure domains: Cloacin immunity protein

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 80.07Å b: 53.72Å c: 36.25Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.218 0.218 0.265
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links

Citations

3 review citations

Immunity proteins: enzyme inhibitors that avoid the active site.
Kleanthous et al. (2001)
2 more

2 mentions without citation

Calculation of accurate small angle X-ray scattering curves from coarse-grained protein models.
Stovgaard et al. (2010)
1 more