› 3egm

X-ray diffraction
2.1Å resolution

Structural basis of iron transport gating in Helicobacter pylori ferritin

Released:
DOI: 10.2210/pdb3egm/pdb
PDB entry 3egm coloured by chain, front view.
PDB entry 3egm coloured by chain, side view.
PDB entry 3egm coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
The crystal structure of ferritin from Helicobacter pylori reveals unusual conformational changes for iron uptake.
Cho KJ, Shin HJ, Lee JH, Kim KJ, Park SS, Lee Y, Lee C, Park SS, Kim KH
J Mol Biol 390 83-98 (2009)
PMID: 19427319

Function and Biology Details

Reaction catalysed: 
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial non-heme ferritin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 181 amino acids
Theoretical weight: 20.92 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZLI1 (Residues: 1-167; Coverage: 100%)
Gene names: ftnA, jhp_0598, pfr
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand FE 15 x FE
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: I4
Unit cell:
a: 128.528Å b: 128.528Å c: 165.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.204
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Self-assembling protein nanoparticles in the design of vaccines.
López-Sagaseta et al. (2016)
4 more

13 mentions without citation

Clinical proteomics identifies potential biomarkers in Helicobacter pylori for gastrointestinal diseases.
Huang et al. (2014)
12 more