› 3eas

X-ray diffraction
2.8Å resolution

Novel dimerization motif in the DEAD box RNA helicase Hera: form 1, complete dimer, asymmetric

Released:
DOI: 10.2210/pdb3eas/pdb
PDB entry 3eas coloured by chain, front view.
PDB entry 3eas coloured by chain, side view.
PDB entry 3eas coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
A novel dimerization motif in the C-terminal domain of the Thermus thermophilus DEAD box helicase Hera confers substantial flexibility.
Klostermeier D, Rudolph MG
Nucleic Acids Res 37 421-30 (2009)
PMID: 19050012

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.87 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 215-426; Coverage: 41%)
Gene name: TT_C1895
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 46.12Å b: 70.77Å c: 181.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.228 0.314
Expression system: Escherichia coli

Quick links

Citations

14 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
13 more

3 mentions without citation

A novel dimerization motif in the C-terminal domain of the Thermus thermophilus DEAD box helicase Hera confers substantial flexibility.
Klostermeier et al. (2009)
2 more