› 3e9n

X-ray diffraction
2.4Å resolution

Crystal structure of a putative short-chain dehydrogenase/reductase from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb3e9n/pdb
PDB entry 3e9n coloured by chain, front view.
PDB entry 3e9n coloured by chain, side view.
PDB entry 3e9n coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Entry authors: Bonanno JB, Gilmore M, Bain KT, Hu S, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenases with different specificities (Related to short-chain alcohol dehydrogenases) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 245 amino acids
Theoretical weight: 26.42 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NMW9 (Residues: 2-235; Coverage: 99%)
Gene name: Cgl2444
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 238.107Å b: 62.26Å c: 127.295Å
α: 90° β: 99.19° γ: 90°
R-values:
R R work R free
0.231 0.229 0.276
Expression system: Escherichia coli

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