› 3dt9

X-ray diffraction
1.85Å resolution

Crystal Structure of Bovin Brain Platelet Activating Factor Acetylhydrolase Covalently Inhibited by Soman

Released:
DOI: 10.2210/pdb3dt9/pdb
PDB entry 3dt9 coloured by chain, front view.
PDB entry 3dt9 coloured by chain, side view.
PDB entry 3dt9 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structures of brain group-VIII phospholipase A2 in nonaged complexes with the organophosphorus nerve agents soman and sarin.
Epstein TM, Samanta U, Kirby SD, Cerasoli DM, Bahnson BJ
Biochemistry 48 3425-35 (2009)
PMID: 19271773

Function and Biology Details

Reaction catalysed: 
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Platelet-activating factor acetylhydrolase IB subunit alpha1 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.89 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29460 (Residues: 1-232; Coverage: 100%)
Gene names: PAFAH1B3, PAFAHG
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

1 bound ligand:
Ligand GD7 1 x GD7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 81.356Å b: 81.356Å c: 72.969Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.21 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Destruction and detection of chemical warfare agents.
Kim et al. (2011)
3 more

3 mentions without citation

Characterization of transport proteins for aromatic compounds derived from lignin: benzoate derivative binding proteins.
Michalska et al. (2012)
2 more