› 3dco

Electron Microscopy
11Å resolution

Drosophila NOD (3DC4) and Bovine Tubulin (1JFF) Docked into the 11-Angstrom Cryo-EM Map of Nucleotide-Free NOD Complexed to the Microtubule

Released:
DOI: 10.2210/pdb3dco/pdb
PDB entry 3dco coloured by chain, front view.
PDB entry 3dco coloured by chain, side view.
PDB entry 3dco coloured by chain, top view.
Source organisms:
Primary publication:
ATPase cycle of the nonmotile kinesin NOD allows microtubule end tracking and drives chromosome movement.
Cochran JC, Sindelar CV, Mulko NK, Collins KA, Kong SE, Hawley RS, Kull FJ
Cell 136 110-22 (2009)
PMID: 19135893
Related structures: EMD-5038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-148293 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein Nod Chain: N
Molecule details ›
Chain: N
Length: 344 amino acids
Theoretical weight: 38.31 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P18105 (Residues: 1-318; Coverage: 48%)
Gene names: CG1763, NODA, nod
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand MG 2 x MG
Ligand ADP 1 x ADP
Ligand ZN 1 x ZN
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 11Å
Relevant EMDB volumes: EMD-5038
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Microtubule tip-interacting proteins: a view from both ends.
Jiang et al. (2011)
16 more

3 mentions without citation

ATPase cycle of the nonmotile kinesin NOD allows microtubule end tracking and drives chromosome movement.
Cochran et al. (2009)
2 more