› 3d8l

X-ray diffraction
2.9Å resolution

Crystal structure of ORF12 from the lactococcus lactis bacteriophage p2

Released:
DOI: 10.2210/pdb3d8l/pdb
PDB entry 3d8l coloured by chain, front view.
PDB entry 3d8l coloured by chain, side view.
PDB entry 3d8l coloured by chain, top view.
Source organism: Lactococcus virus P2
Primary publication:
Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.
Siponen M, Sciara G, Villion M, Spinelli S, Lichière J, Cambillau C, Moineau S, Campanacci V
J Bacteriol 191 728-34 (2009)
PMID: 19047351

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein gp12 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 91 amino acids
Theoretical weight: 10.57 KDa
Source organism: Lactococcus virus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: D3WAD0 (Residues: 1-91; Coverage: 100%)
Sequence domains: Lactococcus lactis phage p2, ORF12
Structure domains: Uncharacterised protein, phage p2 ORF12

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 158.318Å b: 158.318Å c: 99.524Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.253
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structures and host-adhesion mechanisms of lactococcal siphophages.
Spinelli et al. (2014)
2 more

2 mentions without citation

Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.
Siponen et al. (2009)
1 more