› 3d6v

X-ray diffraction
2.2Å resolution

Crystal structure of 4-(trifluoromethyldiazirinyl)phenylalanyl-tRNA synthetase

Released:
DOI: 10.2210/pdb3d6v/pdb
PDB entry 3d6v coloured by chain, front view.
PDB entry 3d6v coloured by chain, side view.
PDB entry 3d6v coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
A genetically encoded diazirine photocrosslinker in Escherichia coli.
Tippmann EM, Liu W, Summerer D, Mack AV, Schultz PG
Chembiochem 8 2210-4 (2007)
PMID: 18000916

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 36.09 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TFQ 1 x TFQ
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 104.155Å b: 104.155Å c: 70.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.27
Expression system: Escherichia coli

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Citations

22 review citations

Adding new chemistries to the genetic code.
Liu et al. (2010)
21 more