› 3cv5

X-ray diffraction
1.6Å resolution

GOLGI MANNOSIDASE II D204A catalytic nucleophile mutant complex with 3alpha,6alpha-mannopentaose

Released:
DOI: 10.2210/pdb3cv5/pdb
PDB entry 3cv5 coloured by chain, front view.
PDB entry 3cv5 coloured by chain, side view.
PDB entry 3cv5 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Golgi alpha-mannosidase II cleaves two sugars sequentially in the same catalytic site.
Shah N, Kuntz DA, Rose DR
Proc Natl Acad Sci U S A 105 9570-5 (2008)
PMID: 18599462

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 68.687Å b: 109.958Å c: 139.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.18
Expression system: Drosophila melanogaster

Quick links

Citations

10 review citations

The repertoire of glycan determinants in the human glycome.
Cummings RD. (2009)
9 more

1 mention without citation

Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties.
Venkatesan et al. (2009)